Introduction: Type 2 Diabetes (T2D) is chronic metabolic disorder that has genetic component. Single Nucleotide Polymorphism (SNP) is the variation single position in DNA sequence among individuals. Glucokinase Regulatory Protein (GCKR) and Potassium Voltage-Gated Channel Subfamily Q (KCNQ1) are SNP genes. The risk or cytosine (C) allele in gene variant GCKR rs780094 and KCNQ1 rs2237892 were associated with incidence of T2D in Asian-Caucasian populations. The aim of this study was to determine frequency of those gene variants and combined genotype in T2D patients in urban area in South Tangerang, Indonesia. Methods: Fifty-four T2D subjects were identified SNP genes using real time Polymerase Chain Reaction techniques. Results: Frequency C allele in GCKR rs780094 was 54.63% and the most common genotype was heterozygous (CT) 46.3%. Frequency of non-risk (T) allele in KCNQ1 rs223792 was 62.03% with the highest homozygous non-risk (TT) genotype 50%. The most common genotype in GCKR and KCNQ1 control group were CT 55%. The highest frequency of combined genotype was CT/TT 25.92%. Conclusion: Gene variant GCKR rs 780094 had more risk allele (C) in T2D patients in urban area in South Tangerang, Indonesia with the most common genotype CT while KCNQ1 rs2237892 had more non-risk alleles (T) with most common genotype TT. The most abundant genotype for GCKR rs780094 and KCNQ1 rs2237892 in control group were CT and the highest frequency of combined genotype was CT/TT.
| Published in | American Journal of Health Research (Volume 10, Issue 4) |
| DOI | 10.11648/j.ajhr.20221004.13 |
| Page(s) | 169-174 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Type 2 Diabetes Mellitus, Variant Gene, Combined Genotype, GCKR rs 780094, KCNQ1 rs223782
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APA Style
Hari Hendarto, Rini Puspitaningrum, Amaliya Mata’ul Hayah, Safira Qalbissilmi, Femmy Nurul Akbar, et al. (2022). Variants and Combined Genotypes Frequencies of GCKR rs780094 and KCNQ1 rs2237892 in Type 2 Diabetes Patients in Indonesia Urban Area. American Journal of Health Research, 10(4), 169-174. https://doi.org/10.11648/j.ajhr.20221004.13
ACS Style
Hari Hendarto; Rini Puspitaningrum; Amaliya Mata’ul Hayah; Safira Qalbissilmi; Femmy Nurul Akbar, et al. Variants and Combined Genotypes Frequencies of GCKR rs780094 and KCNQ1 rs2237892 in Type 2 Diabetes Patients in Indonesia Urban Area. Am. J. Health Res. 2022, 10(4), 169-174. doi: 10.11648/j.ajhr.20221004.13
AMA Style
Hari Hendarto, Rini Puspitaningrum, Amaliya Mata’ul Hayah, Safira Qalbissilmi, Femmy Nurul Akbar, et al. Variants and Combined Genotypes Frequencies of GCKR rs780094 and KCNQ1 rs2237892 in Type 2 Diabetes Patients in Indonesia Urban Area. Am J Health Res. 2022;10(4):169-174. doi: 10.11648/j.ajhr.20221004.13
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Download@article{10.11648/j.ajhr.20221004.13,
author = {Hari Hendarto and Rini Puspitaningrum and Amaliya Mata’ul Hayah and Safira Qalbissilmi and Femmy Nurul Akbar and Chris Adhiyanto},
title = {Variants and Combined Genotypes Frequencies of GCKR rs780094 and KCNQ1 rs2237892 in Type 2 Diabetes Patients in Indonesia Urban Area},
journal = {American Journal of Health Research},
volume = {10},
number = {4},
pages = {169-174},
doi = {10.11648/j.ajhr.20221004.13},
url = {https://doi.org/10.11648/j.ajhr.20221004.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20221004.13},
abstract = {Introduction: Type 2 Diabetes (T2D) is chronic metabolic disorder that has genetic component. Single Nucleotide Polymorphism (SNP) is the variation single position in DNA sequence among individuals. Glucokinase Regulatory Protein (GCKR) and Potassium Voltage-Gated Channel Subfamily Q (KCNQ1) are SNP genes. The risk or cytosine (C) allele in gene variant GCKR rs780094 and KCNQ1 rs2237892 were associated with incidence of T2D in Asian-Caucasian populations. The aim of this study was to determine frequency of those gene variants and combined genotype in T2D patients in urban area in South Tangerang, Indonesia. Methods: Fifty-four T2D subjects were identified SNP genes using real time Polymerase Chain Reaction techniques. Results: Frequency C allele in GCKR rs780094 was 54.63% and the most common genotype was heterozygous (CT) 46.3%. Frequency of non-risk (T) allele in KCNQ1 rs223792 was 62.03% with the highest homozygous non-risk (TT) genotype 50%. The most common genotype in GCKR and KCNQ1 control group were CT 55%. The highest frequency of combined genotype was CT/TT 25.92%. Conclusion: Gene variant GCKR rs 780094 had more risk allele (C) in T2D patients in urban area in South Tangerang, Indonesia with the most common genotype CT while KCNQ1 rs2237892 had more non-risk alleles (T) with most common genotype TT. The most abundant genotype for GCKR rs780094 and KCNQ1 rs2237892 in control group were CT and the highest frequency of combined genotype was CT/TT.},
year = {2022}
}
TY - JOUR T1 - Variants and Combined Genotypes Frequencies of GCKR rs780094 and KCNQ1 rs2237892 in Type 2 Diabetes Patients in Indonesia Urban Area AU - Hari Hendarto AU - Rini Puspitaningrum AU - Amaliya Mata’ul Hayah AU - Safira Qalbissilmi AU - Femmy Nurul Akbar AU - Chris Adhiyanto Y1 - 2022/08/17 PY - 2022 N1 - https://doi.org/10.11648/j.ajhr.20221004.13 DO - 10.11648/j.ajhr.20221004.13 T2 - American Journal of Health Research JF - American Journal of Health Research JO - American Journal of Health Research SP - 169 EP - 174 PB - Science Publishing Group SN - 2330-8796 UR - https://doi.org/10.11648/j.ajhr.20221004.13 AB - Introduction: Type 2 Diabetes (T2D) is chronic metabolic disorder that has genetic component. Single Nucleotide Polymorphism (SNP) is the variation single position in DNA sequence among individuals. Glucokinase Regulatory Protein (GCKR) and Potassium Voltage-Gated Channel Subfamily Q (KCNQ1) are SNP genes. The risk or cytosine (C) allele in gene variant GCKR rs780094 and KCNQ1 rs2237892 were associated with incidence of T2D in Asian-Caucasian populations. The aim of this study was to determine frequency of those gene variants and combined genotype in T2D patients in urban area in South Tangerang, Indonesia. Methods: Fifty-four T2D subjects were identified SNP genes using real time Polymerase Chain Reaction techniques. Results: Frequency C allele in GCKR rs780094 was 54.63% and the most common genotype was heterozygous (CT) 46.3%. Frequency of non-risk (T) allele in KCNQ1 rs223792 was 62.03% with the highest homozygous non-risk (TT) genotype 50%. The most common genotype in GCKR and KCNQ1 control group were CT 55%. The highest frequency of combined genotype was CT/TT 25.92%. Conclusion: Gene variant GCKR rs 780094 had more risk allele (C) in T2D patients in urban area in South Tangerang, Indonesia with the most common genotype CT while KCNQ1 rs2237892 had more non-risk alleles (T) with most common genotype TT. The most abundant genotype for GCKR rs780094 and KCNQ1 rs2237892 in control group were CT and the highest frequency of combined genotype was CT/TT. VL - 10 IS - 4 ER -
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