Cardiovascular diseases (CVD) are one of the leading causes of death and disability, the main cause of which is hyperlipidemia. This work aimed to evaluate the antihyperlipidemic and antioxidant in vivo potential of oil extracted from Sardinella maderensis on rats. The oil was extracted according to the method of Bligh and Dyer and underwent chemical analysis prior to in vivo assays. After studying in vivo the acute toxicity of S. maderensis oil, their antihyperlipidemic was assessed. Twenty-four male Wistar adult rats were randomly divided into four groups of 6 rats each. During a three-week experiment, group 1 was fed with standard laboratory diet (SLD); group 2 received SLD supplemented with boiled egg yolk (5 g/day/rat); group 3 was fed with SLD supplemented with S. maderensis oil (1 g/day/kg of body mass) and group 4 was fed with SLD supplemented with boiled egg yolk and S. maderensis oil. Liver, lung, kidney, adipose tissues and heart were later removed, weighted and analyzed. Some blood biochemical and oxidative stress parameters were also measured. Results showed that S. maderensis oil was siccative, good quality with a lethal dose greater than 5000 mg/Kg of CP and no signs of toxicity were observed. Hyperlipidemic diet increased significantly (p ˂ 0.05) lipid profil, glycemia, uremia, activity of transaminase and γGT, oxidative stress in group 2 compared to other groups. Supplementation with S. maderensis oil significantly (p˂0.05) reduced Lee's index, weight gain and BMI by 8.12%, 26.33% and 19.11% respectively in group 4 compared with group 2. Supplementation with S. maderensis oil decreased significantly (p ˂ 0.05) total-cholesterol, LDL-cholesterol, triglyceride, glycemia, proteinemia and increased levels of HDL-cholesterol in group 4 compared to group 2. Supplementation with S. maderensis oil significantly (p < 0.05) reduced TBARS levels by 54.36% and significantly (p < 0.05) increased GSH levels, SOD, CAT and PON-1 activity by 64.90%, 20.76%, 48.70% and 7.47 respectively in group 4 compared to group 2. This study shows that S. maderensis oil can be used in prevention of hyperlipidemia.
| Published in | Journal of Food and Nutrition Sciences (Volume 12, Issue 1) |
| DOI | 10.11648/j.jfns.20241201.16 |
| Page(s) | 61-71 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Sardinella maderensis, Wistar Rats, Antihyperlipidemia, Oxidative Stress
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APA Style
Koule, J. C. M., Tuem, R. S., Ngangue, R. J. E. M., Dongmo, F. F. D., Nchoutpouen, M. N., et al. (2024). In vivo Antihyperlipidemic and Antioxidant Effect of Oil Extracted from Sardinella maderensis (Lowe, 1838) on Strain Wistar Rats. Journal of Food and Nutrition Sciences, 12(1), 61-71. https://doi.org/10.11648/j.jfns.20241201.16
ACS Style
Koule, J. C. M.; Tuem, R. S.; Ngangue, R. J. E. M.; Dongmo, F. F. D.; Nchoutpouen, M. N., et al. In vivo Antihyperlipidemic and Antioxidant Effect of Oil Extracted from Sardinella maderensis (Lowe, 1838) on Strain Wistar Rats. J. Food Nutr. Sci. 2024, 12(1), 61-71. doi: 10.11648/j.jfns.20241201.16
AMA Style
Koule JCM, Tuem RS, Ngangue RJEM, Dongmo FFD, Nchoutpouen MN, et al. In vivo Antihyperlipidemic and Antioxidant Effect of Oil Extracted from Sardinella maderensis (Lowe, 1838) on Strain Wistar Rats. J Food Nutr Sci. 2024;12(1):61-71. doi: 10.11648/j.jfns.20241201.16
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Download@article{10.11648/j.jfns.20241201.16,
author = {Jules Christophe Manz Koule and Régine Somon Tuem and Roland Jethro Ekwalla Misse Ngangue and Fabrice Fabien Dongho Dongmo and Merlin Ngafon Nchoutpouen and Jean Valery François Nsoga and Mathieu Ndomou and Inocent Gouado},
title = {In vivo Antihyperlipidemic and Antioxidant Effect of Oil Extracted from Sardinella maderensis (Lowe, 1838) on Strain Wistar Rats},
journal = {Journal of Food and Nutrition Sciences},
volume = {12},
number = {1},
pages = {61-71},
doi = {10.11648/j.jfns.20241201.16},
url = {https://doi.org/10.11648/j.jfns.20241201.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20241201.16},
abstract = {Cardiovascular diseases (CVD) are one of the leading causes of death and disability, the main cause of which is hyperlipidemia. This work aimed to evaluate the antihyperlipidemic and antioxidant in vivo potential of oil extracted from Sardinella maderensis on rats. The oil was extracted according to the method of Bligh and Dyer and underwent chemical analysis prior to in vivo assays. After studying in vivo the acute toxicity of S. maderensis oil, their antihyperlipidemic was assessed. Twenty-four male Wistar adult rats were randomly divided into four groups of 6 rats each. During a three-week experiment, group 1 was fed with standard laboratory diet (SLD); group 2 received SLD supplemented with boiled egg yolk (5 g/day/rat); group 3 was fed with SLD supplemented with S. maderensis oil (1 g/day/kg of body mass) and group 4 was fed with SLD supplemented with boiled egg yolk and S. maderensis oil. Liver, lung, kidney, adipose tissues and heart were later removed, weighted and analyzed. Some blood biochemical and oxidative stress parameters were also measured. Results showed that S. maderensis oil was siccative, good quality with a lethal dose greater than 5000 mg/Kg of CP and no signs of toxicity were observed. Hyperlipidemic diet increased significantly (p ˂ 0.05) lipid profil, glycemia, uremia, activity of transaminase and γGT, oxidative stress in group 2 compared to other groups. Supplementation with S. maderensis oil significantly (p˂0.05) reduced Lee's index, weight gain and BMI by 8.12%, 26.33% and 19.11% respectively in group 4 compared with group 2. Supplementation with S. maderensis oil decreased significantly (p ˂ 0.05) total-cholesterol, LDL-cholesterol, triglyceride, glycemia, proteinemia and increased levels of HDL-cholesterol in group 4 compared to group 2. Supplementation with S. maderensis oil significantly (p S. maderensis oil can be used in prevention of hyperlipidemia.
},
year = {2024}
}
TY - JOUR T1 - In vivo Antihyperlipidemic and Antioxidant Effect of Oil Extracted from Sardinella maderensis (Lowe, 1838) on Strain Wistar Rats AU - Jules Christophe Manz Koule AU - Régine Somon Tuem AU - Roland Jethro Ekwalla Misse Ngangue AU - Fabrice Fabien Dongho Dongmo AU - Merlin Ngafon Nchoutpouen AU - Jean Valery François Nsoga AU - Mathieu Ndomou AU - Inocent Gouado Y1 - 2024/02/21 PY - 2024 N1 - https://doi.org/10.11648/j.jfns.20241201.16 DO - 10.11648/j.jfns.20241201.16 T2 - Journal of Food and Nutrition Sciences JF - Journal of Food and Nutrition Sciences JO - Journal of Food and Nutrition Sciences SP - 61 EP - 71 PB - Science Publishing Group SN - 2330-7293 UR - https://doi.org/10.11648/j.jfns.20241201.16 AB - Cardiovascular diseases (CVD) are one of the leading causes of death and disability, the main cause of which is hyperlipidemia. This work aimed to evaluate the antihyperlipidemic and antioxidant in vivo potential of oil extracted from Sardinella maderensis on rats. The oil was extracted according to the method of Bligh and Dyer and underwent chemical analysis prior to in vivo assays. After studying in vivo the acute toxicity of S. maderensis oil, their antihyperlipidemic was assessed. Twenty-four male Wistar adult rats were randomly divided into four groups of 6 rats each. During a three-week experiment, group 1 was fed with standard laboratory diet (SLD); group 2 received SLD supplemented with boiled egg yolk (5 g/day/rat); group 3 was fed with SLD supplemented with S. maderensis oil (1 g/day/kg of body mass) and group 4 was fed with SLD supplemented with boiled egg yolk and S. maderensis oil. Liver, lung, kidney, adipose tissues and heart were later removed, weighted and analyzed. Some blood biochemical and oxidative stress parameters were also measured. Results showed that S. maderensis oil was siccative, good quality with a lethal dose greater than 5000 mg/Kg of CP and no signs of toxicity were observed. Hyperlipidemic diet increased significantly (p ˂ 0.05) lipid profil, glycemia, uremia, activity of transaminase and γGT, oxidative stress in group 2 compared to other groups. Supplementation with S. maderensis oil significantly (p˂0.05) reduced Lee's index, weight gain and BMI by 8.12%, 26.33% and 19.11% respectively in group 4 compared with group 2. Supplementation with S. maderensis oil decreased significantly (p ˂ 0.05) total-cholesterol, LDL-cholesterol, triglyceride, glycemia, proteinemia and increased levels of HDL-cholesterol in group 4 compared to group 2. Supplementation with S. maderensis oil significantly (p S. maderensis oil can be used in prevention of hyperlipidemia. VL - 12 IS - 1 ER -
Laboratory of Food Sciences and Nutrition, Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
